Efficient evaluation of a gene containment system for poplar through early flowering induction.
Identifieur interne : 000436 ( Main/Exploration ); précédent : 000435; suivant : 000437Efficient evaluation of a gene containment system for poplar through early flowering induction.
Auteurs : M Valentina Briones [Argentine] ; Hans Hoenicka [Allemagne] ; Luis A. Ca As [Espagne] ; José Pío Beltrán [Espagne] ; Dieter Hanelt [Allemagne] ; Sandra Sharry [Argentine] ; Matthias Fladung [Allemagne]Source :
- Plant cell reports [ 1432-203X ] ; 2020.
Abstract
KEY MESSAGE
The early flowering system HSP::AtFT allowed a fast evaluation of a gene containment system based on the construct PsEND1::barnase-barstar for poplar. Transgenic lines showed disturbed pollen development and sterility. Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase-barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
DOI: 10.1007/s00299-020-02515-1
PubMed: 32052127
PubMed Central: PMC7165154
Affiliations:
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Ca As</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Beltran, Jose Pio" sort="Beltran, Jose Pio" uniqKey="Beltran J" first="José Pío" last="Beltrán">José Pío Beltrán</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Hanelt, Dieter" sort="Hanelt, Dieter" uniqKey="Hanelt D" first="Dieter" last="Hanelt">Dieter Hanelt</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Pflanzenwissenschaften und Mikrobiologie, Universität Hamburg, 22609, Hamburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenwissenschaften und Mikrobiologie, Universität Hamburg, 22609, Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Sharry, Sandra" sort="Sharry, Sandra" uniqKey="Sharry S" first="Sandra" last="Sharry">Sandra Sharry</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Investigaciones de la Madera (LIMAD), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, B1900, La Plata, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Investigaciones de la Madera (LIMAD), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, B1900, La Plata</wicri:regionArea><wicri:noRegion>La Plata</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>CIT-Viedma, Universidad Nacional de Río Negro, R8500, Viedma, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CIT-Viedma, Universidad Nacional de Río Negro, R8500, Viedma</wicri:regionArea><wicri:noRegion>Viedma</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), B1900, La Plata, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), B1900, La Plata</wicri:regionArea><wicri:noRegion>La Plata</wicri:noRegion></affiliation></author><author><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b></p><p>The early flowering system HSP::AtFT allowed a fast evaluation of a gene containment system based on the construct PsEND1::barnase-barstar for poplar. Transgenic lines showed disturbed pollen development and sterility. Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase-barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32052127</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Efficient evaluation of a gene containment system for poplar through early flowering induction.</ArticleTitle><Pagination><MedlinePgn>577-587</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-020-02515-1</ELocationID><Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">The early flowering system HSP::AtFT allowed a fast evaluation of a gene containment system based on the construct PsEND1::barnase-barstar for poplar. Transgenic lines showed disturbed pollen development and sterility. Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase-barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Briones</LastName><ForeName>M Valentina</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, B1900, La Plata, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), B1900, La Plata, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoenicka</LastName><ForeName>Hans</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. hans.hoenicka@thuenen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cañas</LastName><ForeName>Luis A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Beltrán</LastName><ForeName>José Pío</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022, Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hanelt</LastName><ForeName>Dieter</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institut für Pflanzenwissenschaften und Mikrobiologie, Universität Hamburg, 22609, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sharry</LastName><ForeName>Sandra</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigaciones de la Madera (LIMAD), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, B1900, La Plata, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CIT-Viedma, Universidad Nacional de Río Negro, R8500, Viedma, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), B1900, La Plata, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fladung</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9301-8581</Identifier><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>57048249</GrantID><Agency>Deutscher Akademischer Austauschdienst</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biosafety</Keyword><Keyword MajorTopicYN="N">Early flowering</Keyword><Keyword MajorTopicYN="N">Gene containment</Keyword><Keyword MajorTopicYN="N">Genetic transformation</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">PsEND1</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>29</Day></PubMedPubDate><PubMedPubDate 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IdType="pubmed">20811751</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Argentine</li><li>Espagne</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="Argentine"><noRegion><name sortKey="Briones, M Valentina" sort="Briones, M Valentina" uniqKey="Briones M" first="M Valentina" last="Briones">M Valentina Briones</name></noRegion><name sortKey="Briones, M Valentina" sort="Briones, M Valentina" uniqKey="Briones M" first="M Valentina" last="Briones">M Valentina Briones</name><name sortKey="Sharry, Sandra" sort="Sharry, Sandra" uniqKey="Sharry S" first="Sandra" last="Sharry">Sandra Sharry</name><name sortKey="Sharry, Sandra" sort="Sharry, Sandra" uniqKey="Sharry S" first="Sandra" last="Sharry">Sandra Sharry</name><name sortKey="Sharry, Sandra" sort="Sharry, Sandra" uniqKey="Sharry S" first="Sandra" last="Sharry">Sandra Sharry</name></country><country name="Allemagne"><noRegion><name sortKey="Hoenicka, Hans" sort="Hoenicka, Hans" uniqKey="Hoenicka H" first="Hans" last="Hoenicka">Hans Hoenicka</name></noRegion><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><name sortKey="Hanelt, Dieter" sort="Hanelt, Dieter" uniqKey="Hanelt D" first="Dieter" last="Hanelt">Dieter Hanelt</name></country><country name="Espagne"><noRegion><name sortKey="Ca As, Luis A" sort="Ca As, Luis A" uniqKey="Ca As L" first="Luis A" last="Ca As">Luis A. Ca As</name></noRegion><name sortKey="Beltran, Jose Pio" sort="Beltran, Jose Pio" uniqKey="Beltran J" first="José Pío" last="Beltrán">José Pío Beltrán</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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